In the last years, Th17 lymphocytes and regulatory B lymphocytes (Breg) seem to play a promising role in understanding the pathogenesis of autoimmune disorders. In isolated Hashimoto’s thyroiditis (HT), increased Th1 and Th17 activation has been described but little is known about Breg which allegedly suppress the pro-inflammatory response. Aim of this study was to compare Th17 and Breg subpopulation in healthy subjects and in patients affected by HT. A total of 19 patients (17 women and 2 men, mean age 48 – 10 years) affected by HT and 18 age- and sex-matched healthy donors (16 women and 2 men, mean age 42 – 12 years) were enrolled in this study. Effector Th17 lymphocytes were obtained from overnight stimulated PBMCs, then stained, fixed, permeabilized and stained intracellularly with anti-IL-17A. For Breg detection, freshly PBMCs were surface stained with anti-CD19, anti-CD24, anti-CD38, and anti-CD27. Cells were then acquired on a FACS ARIA II. Increased percentage (2.6% – 1.6 versus 1.6% – 0.9; p = 0.0337) of Th17 cells in HT patients as compared with healthy controls were observed. Instead, we found similar percentage of non stimulated CD24hiCD38hi Breg cells between isolated HT patients and healthy donors (2.4% – 0.9 versus 2.0% – 0.7; p = ns). Also, mature CD24intCD38int and memory CD24hiCD38neg B subsets, were similar in HT patients and healthy controls (30.8 vs. 25.1% and 49.5 vs. 56.0%, respectively; p = ns). Following CpG oligonucleotide stimulation (functional assay of B cells), small but not significant differences were seen in total CD19+ cells subset and total B lymphocytes producing IL-10 between HT patients and healthy donors (2.9% – 1.9 and 1.9% – 1.1; p = ns). However, a significant increase was recognized in the percentage of IL10-producing Breg cells in HT patients as compared to healthy controls (3.9% – 1.8 and 2.4% – 1.1; p = 0.0303). These preliminary findings confirmed activation of proinflammatory Th17 pathway but described for the first time an upregulation of IL-10 producing Breg cells in Hashimoto’s thyroiditis which may oppose to Th17 activation.
CHARACTERIZATION OF CD4+TH17 AND B REGULATORY CELLS IN HASHIMOTO’S THYROIDITIS / Santaguida, M.; Gatto, I.; Virili, C.; Mangino, G.; Stramazzo, I.; Brusca, N.; Cellini, M.; Gargano, L.; Centanni, M.. - STAMPA. - (2016). (Intervento presentato al convegno 86th Annual Meeting of the American Thyroid Association tenutosi a Denver).
CHARACTERIZATION OF CD4+TH17 AND B REGULATORY CELLS IN HASHIMOTO’S THYROIDITIS
M. Santaguida
;I. Gatto
;C. Virili
;G. Mangino
;I. Stramazzo
;N. Brusca
;M. Cellini
;M. Centanni
2016
Abstract
In the last years, Th17 lymphocytes and regulatory B lymphocytes (Breg) seem to play a promising role in understanding the pathogenesis of autoimmune disorders. In isolated Hashimoto’s thyroiditis (HT), increased Th1 and Th17 activation has been described but little is known about Breg which allegedly suppress the pro-inflammatory response. Aim of this study was to compare Th17 and Breg subpopulation in healthy subjects and in patients affected by HT. A total of 19 patients (17 women and 2 men, mean age 48 – 10 years) affected by HT and 18 age- and sex-matched healthy donors (16 women and 2 men, mean age 42 – 12 years) were enrolled in this study. Effector Th17 lymphocytes were obtained from overnight stimulated PBMCs, then stained, fixed, permeabilized and stained intracellularly with anti-IL-17A. For Breg detection, freshly PBMCs were surface stained with anti-CD19, anti-CD24, anti-CD38, and anti-CD27. Cells were then acquired on a FACS ARIA II. Increased percentage (2.6% – 1.6 versus 1.6% – 0.9; p = 0.0337) of Th17 cells in HT patients as compared with healthy controls were observed. Instead, we found similar percentage of non stimulated CD24hiCD38hi Breg cells between isolated HT patients and healthy donors (2.4% – 0.9 versus 2.0% – 0.7; p = ns). Also, mature CD24intCD38int and memory CD24hiCD38neg B subsets, were similar in HT patients and healthy controls (30.8 vs. 25.1% and 49.5 vs. 56.0%, respectively; p = ns). Following CpG oligonucleotide stimulation (functional assay of B cells), small but not significant differences were seen in total CD19+ cells subset and total B lymphocytes producing IL-10 between HT patients and healthy donors (2.9% – 1.9 and 1.9% – 1.1; p = ns). However, a significant increase was recognized in the percentage of IL10-producing Breg cells in HT patients as compared to healthy controls (3.9% – 1.8 and 2.4% – 1.1; p = 0.0303). These preliminary findings confirmed activation of proinflammatory Th17 pathway but described for the first time an upregulation of IL-10 producing Breg cells in Hashimoto’s thyroiditis which may oppose to Th17 activation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
